Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure-function analysis of the human papillomavirus type 16 E7 oncoprotein.

W C Phelps1, K Münger, C L Yee

  • 1Division of Virology, Burroughs Wellcome Co., Research Triangle Park, North Carolina 27709.

Journal of Virology
|April 1, 1992
PubMed
Summary

Human papillomavirus type 16 E7 protein’s transforming and trans-activating domains overlap, requiring retinoblastoma protein (pRB) binding for function. Separable functions of E7 indicate distinct roles in cellular transformation and gene regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tracheotomy in Croup.

Buffalo medical and surgical journal·2023
Same author

Abstract of the Proceedings of the Buffalo Medical Association.

Buffalo medical and surgical journal·2023
Same author

Abstract of the Proceedings of Buffalo Medical Association.

Buffalo medical and surgical journal·2023
Same author

Abstract of the Proceedings of the Buffalo Medical Association.

Buffalo medical and surgical journal·2023
Same author

Alemtuzumab, total skin electron beam, and non-myeloablative allogeneic haematopoietic stem-cell transplantation in advanced sezary syndrome: a retrospective cohort study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Radiation Safety Aspects of Iodine-131 metaiodobenzylguanidine (131I mIBG) Therapy Program Startup.

Health physics·2020

Area of Science:

  • Molecular Biology
  • Oncogenesis
  • Virology

Background:

  • Human papillomavirus type 16 (HPV16) E7 protein is a nuclear phosphoprotein with functions similar to adenovirus E1A and papovavirus T antigens.
  • E7 protein is known to cooperate with ras oncogenes, transactivate promoters, and affect growth factor signaling, potentially through interaction with the retinoblastoma tumor suppressor protein (pRB).

Purpose of the Study:

  • To investigate the functional domains of the HPV16 E7 protein.
  • To determine the role of pRB association in E7's transforming and trans-activating activities.
  • To elucidate the relationship between E7's cellular transformation and trans activation functions.

Main Methods:

  • Construction and analysis of site-specific mutants of the HPV16 E7 gene.

Related Experiment Videos

  • Assessment of mutant E7 proteins for intracellular stability, ras cooperativity, and transcriptional trans activation.
  • Evaluation of pRB association and phosphorylation status of E7 mutants.

    • Main Results:

    • The transforming and trans-activating domains of E7 significantly overlap within a region analogous to adenovirus E1A conserved region 2.
    • pRB binding is essential for both the transforming and trans-activating functions of E7.
    • Deletion of sequences in conserved region 1 abolished cellular transformation but only marginally affected trans activation.

    Conclusions:

    • E7 protein's cellular transformation and trans activation functions are interrelated but separable.
    • pRB binding is a critical determinant for E7's oncogenic activities.
    • Specific regions of E7, particularly conserved region 1 and 2, play distinct roles in its multifaceted functions.