Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glucocorticoids decrease vitamin D receptor number and gene expression in human osteosarcoma cells.

M Godschalk1, J R Levy, R W Downs

  • 1Department of Internal Medicine, McGuire VA Medical Center, Richmond, Virginia.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|January 11, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-reported calcium use in a cohort of postmenopausal women receiving osteoporosis therapy: results from POSSIBLE US™.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2015
Same author

Advancement of Medicine and the Medical Profession.

Journal of the National Medical Association·2010
Same author

Effect of weight loss by a low-fat diet and a low-carbohydrate diet on peptide YY levels.

International journal of obesity (2005)·2010
Same author

Persistence and switching patterns among women with varied osteoporosis medication histories: 12-month results from POSSIBLE US.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2010
Same author

Effects of arzoxifene on bone, lipid markers, and safety parameters in postmenopausal women with low bone mass.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2009
Same author

Antagonism of gamma-hydroxybutyrate-induced hypersynchronization in the ECoG of the rat by anti-petit mal drugs.

Neuroscience letters·2009
Same journal

Ossification of the Posterior Longitudinal Ligament - Novel findings from Pandora's box of ENPP1 Deficiency.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same journal

From loci to cells: mapping variant function in bone and joint diseases.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same journal

The Journal of Bone and Mineral Research (JBMR) 40th anniversary celebration: the fourth decade (Part 2).

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same journal

Increased Prevalence of Coronary Artery Calcification in Patients with Post-Surgical Hypoparathyroidism.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same journal

Alendronate for 6 or 12 months following denosumab discontinuation in women with postmenopausal osteoporosis: A prospective observational study.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same journal

Is Zoledronic acid cost-saving in daily practice?

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
See all related articles

Glucocorticoids like dexamethasone reduce vitamin D receptor (VDR) number and mRNA levels in osteosarcoma cells. This decrease in VDR is likely due to reduced gene transcription or mRNA processing, not altered mRNA stability.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Glucocorticoids (GC) are known to inhibit some vitamin D actions.
  • Evidence suggests GC influence vitamin D receptor (VDR) levels.
  • The precise mechanisms of GC inhibition on vitamin D are not fully understood.

Purpose of the Study:

  • To investigate the effects of dexamethasone (DEX) on VDR number and mRNA in MG-63 human osteosarcoma cells.
  • To elucidate the mechanism by which DEX alters VDR expression.

Main Methods:

  • MG-63 cells were treated with DEX.
  • VDR number and binding affinity for 1,25-(OH)2D3 were measured.
  • VDR mRNA levels and stability were assessed following DEX treatment.

Related Experiment Videos

Main Results:

  • DEX significantly decreased VDR number by approximately 70% without altering 1,25-(OH)2D3 binding affinity.
  • DEX treatment resulted in a time- and dose-dependent reduction in VDR mRNA.
  • DEX did not significantly affect the half-life of VDR mRNA, indicating stability was maintained.

Conclusions:

  • Dexamethasone reduces both VDR number and VDR mRNA in MG-63 cells.
  • The observed decrease in VDR mRNA is likely mediated by inhibited VDR gene transcription or altered mRNA processing.
  • Glucocorticoids impact vitamin D signaling pathways by modulating VDR expression.