Related Experiment Videos
Antisense inhibition of oncogene expression.
L Neckers1, L Whitesell, A Rosolen
1Clinical Pharmacology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Critical Reviews in Oncogenesis
|January 1, 1992
Summary
Antisense technology uses specific oligonucleotides to inhibit gene expression in living cells. This approach aids in understanding gene roles in cell growth and differentiation.
Area of Science:
- Molecular Biology
- Cell Biology
- Biotechnology
Background:
- Studying gene function requires specific interference with gene expression or protein activity.
- Inhibiting intracellular proteins in living cells is challenging.
- Monoclonal antibodies are effective for cell surface proteins but not intracellular targets.
Purpose of the Study:
- To review the principles and applications of antisense technology for inhibiting specific gene expression.
- To discuss challenges such as cellular uptake and intracellular distribution.
- To explore the biological effects of antisense administration in studying gene regulation.
Main Methods:
- Review of antisense technology principles.
- Discussion of oligonucleotide derivatives and their properties.
- Presentation of biological examples using antisense administration.
Main Results:
- Antisense technology offers a method to specifically inhibit gene expression in viable cells.
- Challenges in cellular uptake and intracellular distribution of antisense agents were addressed.
- Examples demonstrate the utility of antisense in studying gene regulation of cell growth and differentiation.
Conclusions:
- Antisense technology is a valuable tool for investigating the roles of specific genes.
- Further development of oligonucleotide derivatives can improve efficacy and delivery.
- This technology facilitates research into cell growth and differentiation processes.