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Updated: Aug 19, 2026
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
Published on: June 25, 2014
[Experimental study of amylin-induced functional damage to rat pancreatic islet cells in vitro]
1Department of Endocrinology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To investigate the effect of amylin on the functions of pancreatic islet cells in vitro.
Methods:
In vitro cultured pancreatic islet cells from neonatal rats were used for studying the effects of amylin at different concentrations on insulin secretion, and DNA content and insulin contents in the cells.
Results:
Insulin secretion was significantly inhibited when amylin concentration was higher than 10 micromol/L (P < 0.01). After incubation with 10 micromol/L amylin for 2 h, insulin secretion of islet cells was dose-dependently inhibited by to 16.7 mmol/L glucose stimulation (P < 0.01). The DNA and insulin contents in the cells rose significantly when amylin concentration was higher than 10 micromol/L as compared with the control group (P < 0.01).
Conclusion:
Amylin at concentrations higher than 10 micromol/L can remarkably inhibit insulin secretion and release induced by 16.7 mmol/L glucose stimulation.
