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Structural and functional characterization of the poliovirus replication complex.

K Bienz1, D Egger, T Pfister

  • 1Institute for Medical Microbiology, University of Basel, Switzerland.

Journal of Virology
|May 1, 1992
PubMed
Summary
This summary is machine-generated.

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Poliovirus replication complexes form a unique structure with compact membranes and surrounding vesicles. New RNA strands are released from these complexes upon completion.

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Poliovirus replication involves complex molecular machinery within infected cells.
  • Understanding the structural organization of poliovirus replication complexes is crucial for deciphering viral RNA synthesis.

Purpose of the Study:

  • To isolate and characterize membrane-bound poliovirus replication complexes.
  • To elucidate the structural architecture and RNA synthesis capabilities of these complexes.

Main Methods:

  • Isolation of replication complexes from poliovirus-infected HEp-2 cells using sucrose gradient centrifugation.
  • Ultrastructural analysis of isolated complexes.
  • In vitro RNA synthesis assays.
  • In situ hybridization for RNA localization.

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Main Results:

  • Two distinct populations of replication complexes were identified, both featuring a rosette-like shell of vesicles and compact internal membranes.
  • These complexes exhibit in vitro RNA synthesizing capacity, producing 36S plus-strand RNA.
  • Disruption of membranes inhibited RNA completion but allowed elongation, suggesting membrane involvement in RNA release.
  • Progeny RNA strands are released upon completion at specific contact sites between compact membranes and surrounding vesicles.

Conclusions:

  • The poliovirus replication complex possesses a defined architecture with nascent RNA enclosed within compact membranes and replication forks protected by protein.
  • RNA release is a regulated process occurring at the interface of internal and external membranes within the replication complex.