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Nuclear location of phosphoglycerate mutase BB isozyme in rat tissues
1Unitat de Bioquimica, Facultat de Medicina, Universitat de Barcelona, Spain.
Abstract:
We have previously reported (Ureña et al. Eur. J. Cell Biol. 1990) that in skeletal muscle, type MM phosphoglycerate mutase isozyme is present in the nucleus as well as in the cytosol. To determine whether type BB phosphoglycerate mutase isozyme is also present in nucleus, the subcellular location of this isozyme was studied in different rat tissues by cell fractionation and immunogold techniques. With the aid of high affinity-purified anti-phosphoglycerate mutase BB isozyme antibodies, the isozyme was located in the nucleus of neuronal, astroglial and liver cells but not in the nucleus of oligodendroglial and endothelial cells. Biochemical studies on purified nuclear fractions also demonstrated the presence of phosphoglycerate mutase activity in the nucleus. Both immunocytochemical and biochemical techniques showed that nuclear phosphoglycerate mutase-specific activity depended on the type of cell.
Insights
The BB phosphoglycerate mutase isozyme is found in the nucleus of neuronal, astroglial, and liver cells, but not oligodendroglial or endothelial cells. Nuclear phosphoglycerate mutase activity varies by cell type.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Previous research indicated the presence of type MM phosphoglycerate mutase isozyme in the nucleus and cytosol of skeletal muscle cells.
- The subcellular localization of the type BB phosphoglycerate mutase isozyme remained uncharacterized in various cell types.
Purpose of the Study:
- To investigate the nuclear presence of the type BB phosphoglycerate mutase isozyme in different rat tissues.
- To determine if the subcellular distribution of phosphoglycerate mutase isozymes is cell-type specific.
Main Methods:
- Cell fractionation techniques were employed to isolate cellular components.
- Immunogold labeling with high-affinity antibodies against the BB phosphoglycerate mutase isozyme was used for subcellular localization.
- Biochemical assays were performed on purified nuclear fractions to quantify enzyme activity.
Main Results:
- The BB phosphoglycerate mutase isozyme was detected in the nucleus of neuronal, astroglial, and liver cells.
- The isozyme was notably absent from the nucleus of oligodendroglial and endothelial cells.
- Biochemical analysis confirmed phosphoglycerate mutase activity within purified nuclear fractions.
- Nuclear phosphoglycerate mutase-specific activity was found to be dependent on the cell type.
Conclusions:
- The type BB phosphoglycerate mutase isozyme exhibits cell-type specific nuclear localization in rat tissues.
- These findings suggest distinct roles for different phosphoglycerate mutase isozymes in nuclear functions across various cell types.