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Related Experiment Video

Updated: Jul 13, 2026

Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester
08:10

Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester

Published on: July 7, 2015

Lectin staining of sheep microglia

N A Pennell1, S D Hurley, W J Streit

  • 1Department of Neuroscience, JHMHC, University of Florida, Gainesville 32610.

Histochemistry
|December 1, 1994
PubMed
Summary

A modified lectin staining method effectively labels resting microglia and perivascular cells in sheep brain tissue. This technique enhances visualization of these crucial cell types in neuropathology research.

Area of Science:

  • Neuroscience
  • Histology
  • Immunohistochemistry

Background:

  • Microglia are key immune cells in the brain.
  • Griffonia simplicifolia isolectin B4 (IB4) is a known microglial stain.
  • Existing methods may not optimally stain resting microglia or perivascular cells.

Purpose of the Study:

  • To modify the IB4 lectin staining method.
  • To improve staining of resting microglia and perivascular cells.
  • To apply the method to vibratome sections of normal sheep brain.

Main Methods:

  • Utilized formaldehyde or paraformaldehyde fixation.
  • Incorporated Triton X-100 incubation before staining.
  • Applied the modified lectin staining to sheep brain vibratome sections.

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Main Results:

  • Successfully stained resting microglia.
  • Successfully stained perivascular cells.
  • Demonstrated efficacy in normal sheep brain tissue.

Conclusions:

  • The modified IB4 lectin staining method is effective for visualizing resting microglia and perivascular cells.
  • This technique offers improved diagnostic potential in neuropathology.
  • The method is suitable for fixed vibratome sections of brain tissue.