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In vitro glycated low-density lipoprotein interaction with human monocyte-derived macrophages
A Gugliucci Creriche1, S Dumont, J C Siffert
1Biochemistry Laboratory, Faculté de Pharmacie, Université Louis Pasteur, Illkirch, France.
Research in Immunology
|January 1, 1992
Abstract:
Human low-density lipoprotein (LDL) was glycated in vitro (5 days, glucose 50 mmol/l), labelled with 125I, and its binding and uptake by human monocyte-derived macrophages studied. Glycation produced lower binding and lower uptake. Competition experiments using unlabelled LDL (control, glycated, and acetyl-LDL) showed that most glycated LDL was taken up by the apolipoprotein-B100: E receptor pathway. Results suggest that less of the glycated LDL may enter the cells via scavenger receptors, and very minute amount via non-saturable receptor-independent pathways.