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Related Experiment Videos

Brain cell membrane dysfunction following acute asphyxia in newborn piglets.

J M Goplerud1, O P Mishra, M Delivoria-Papadopoulos

  • 1Division of Neonatal-Perinatal Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104.

Biology of the Neonate
|January 1, 1992
PubMed
Summary

Brain cell membrane damage occurs after asphyxia in newborn piglets. Enzyme activity decreases and lipid peroxidation increases during recovery, indicating cellular damage from oxygen free radicals.

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Area of Science:

  • Neuroscience
  • Biochemistry
  • Perinatal Medicine

Background:

  • Asphyxia can cause brain injury in newborns.
  • Cell membrane integrity is crucial for brain function.
  • The effects of repeated asphyxia on brain cell membranes are not fully understood.

Purpose of the Study:

  • To investigate brain cell membrane function during and after single and repeated asphyxia episodes in newborn piglets.
  • To assess the impact of asphyxia on cerebral Na+,K(+)-ATPase activity and lipid peroxidation.
  • To determine if recovery from asphyxia leads to cellular membrane damage.

Main Methods:

  • Newborn piglets were subjected to single or repeated asphyxia episodes.
  • Cerebral Na+,K(+)-ATPase activity was measured.

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  • Lipid peroxidation products (conjugated dienes, fluorescent compounds) were quantified.
  • Measurements were taken during control, asphyxia, and recovery phases.

    • Main Results:

    • Cerebral Na+,K(+)-ATPase activity remained unchanged during asphyxia but significantly decreased during recovery.
    • Lipid peroxidation products increased during recovery from both single and repeated asphyxia.
    • A 73% and 82% reduction in enzyme activity was observed after single and repeated asphyxias, respectively.

    Conclusions:

    • Asphyxia causes significant brain cell membrane damage in newborn piglets.
    • Decreased Na+,K(+)-ATPase activity and increased lipid peroxidation indicate cellular damage during recovery.
    • Oxygen free radical formation during recovery is implicated in this membrane damage.