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Updated: Aug 10, 2026

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
Published on: February 26, 2011
Proofreading, NTPases and translation: constraints on accurate biochemistry
1Department of MCD Biology, University of Colorado, Boulder 80309-0347.
Abstract:
Two accurate individual reactions can work together as a more accurate overall mechanism. This is straightforward for a transient reaction, but the same accuracy in the steady state (termed proofreading) requires many preconditions. The preconditions for proofreading can be summarized as four statements, which allow experimental identification and subsequent confirmation of proofreading mechanisms.
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Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
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