[Determination of restriction endonucleases in Streptomyces and Nocardia colonies]

A simple technique is proposed for the detection of restriction endonucleases in Streptomyces and Nocardia cells. The analysis was performed directly in the cells collected from colonies cultivated on Petri dishes with an inoculation loop. The cells were treated with lysozyme, EDTA and Triton X-100. The lysates were tested for restriction endonucleases. The technique enables the detection of enzymes Nco I, Not I, Nru I, Sfr 3031, and Sfi I in the lysates of the respective strains-producers.