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Development of mouse oocytes superovulated at different ages
F Sugiyama1, N Kajiwara, S Hayashi
1Laboratory Animal Research Center, University of Tsukuba, Ibaraki, Japan.
Laboratory Animal Science
|June 1, 1992
Summary
Younger mice provide the best oocytes for research. Superovulated 25-day-old mice yield the most viable oocytes for producing transgenic mice efficiently.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Genetics
Background:
- Superovulation is a key technique in assisted reproduction and genetic engineering.
- Understanding the impact of donor age on oocyte quality is crucial for optimizing experimental outcomes.
Purpose of the Study:
- To evaluate the influence of donor mouse age (25, 50, or 90 days) on oocyte yield and developmental potential.
- To determine the most economical and efficient age for sourcing superovulated oocytes for transgenic mouse production.
Main Methods:
- Superovulation was induced in four mouse strains (C57BL/6N, DBA/2N, ICR, B6D2F1) at different ages.
- Oocytes were collected, subjected to in vitro fertilization (IVF), and cultured.
- Two-cell stage embryos were transferred to pseudopregnant recipients for gestation and development.
Main Results:
- The highest number of oocytes were recovered from superovulated 25-day-old mice across all strains.
- Approximately 80% of oocytes reached the two-cell stage after IVF.
- Sixty percent of viable oocytes developed to the weanling stage post-transfer, indicating donor age did not significantly affect oocyte viability.
Conclusions:
- Donor mouse age (25, 50, or 90 days) does not impact the viability of superovulated oocytes.
- Superovulated 25-day-old mice are an economical and efficient source for obtaining viable oocytes.
- This finding supports the use of young mice for optimizing transgenic mouse production.