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Slow time-dependent cellular transformation induced at restrictive temperature by ts-src mutants.
T David-Pfeuty1, Y Nouvian-Dooghe
1Institut Curie-Biologie, Centre Universitaire, Orsay, France.
Oncogene
|August 1, 1992
Summary
Temperature-sensitive Rous sarcoma virus infection of cells reveals that the non-permissive temperature leads to a transient normal state that drifts into a distinct transformed state (T2). This occurs with pp60v-src accumulation, suggesting two transformation pathways.
Area of Science:
- Oncology
- Virology
- Cell Biology
Background:
- Temperature-sensitive Rous sarcoma virus (tsRSV) is used to study cell transformation dynamics.
- Cells infected with tsRSV are typically considered normal at non-permissive temperatures and transformed at permissive temperatures.
Purpose of the Study:
- To investigate the temporal dynamics of cell transformation induced by tsRSV.
- To determine if the non-permissive temperature represents a stable normal state or a transient phase.
Main Methods:
- Utilized tsPA1-infected FR3T3 cells cultured at restrictive and permissive temperatures.
- Monitored morphological and growth characteristics of cells over time.
- Assessed the intracellular localization and accumulation of pp60v-src.
Main Results:
- Cells at the restrictive temperature entered a transient, drifting state, not a stable normal state.
- This transient state evolved into a distinct transformed state (T2) with unique properties.
- pp60v-src accumulated near the nucleus at the restrictive temperature, with release to the plasma membrane upon shifting to permissive temperature.
Conclusions:
- The v-src protein may induce cell transformation through two distinct pathways, leading to different transformed states (T1 and T2).
- The temperature-dependent transformation suggests a complex regulatory mechanism for v-src.
- Further research is needed to elucidate the mechanisms of time-dependent transformation by ts-src mutants.