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Related Experiment Videos

Conjugative plasmids in Bordetella avium.

J S Morris1, G H Luginbuhl

  • 1Department of Microbiology, North Carolina State University, Raleigh 27695-7615.

Plasmid
|May 1, 1992
PubMed
Summary

Three Bordetella avium antibiotic-resistant plasmids were conjugative. Mapping revealed resistance genes clustered with restriction sites, while conjugation genes were in separate regions on plasmid p4093.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Antibiotic resistance in bacteria poses a significant threat to public health.
  • Bordetella avium is an important pathogen in veterinary medicine, particularly in poultry.
  • Understanding the genetic basis of antibiotic resistance and plasmid transfer in B. avium is crucial for controlling infections.

Purpose of the Study:

  • To investigate the characteristics of antibiotic-resistant plasmids in virulent Bordetella avium strains.
  • To physically and genetically map a conjugative plasmid (p4093) from B. avium.
  • To identify the genetic regions responsible for antibiotic resistance and conjugative transfer.

Main Methods:

  • Isolation and characterization of antibiotic-resistant plasmids from Bordetella avium.
  • Conjugation experiments to determine plasmid transferability.
  • Physical and genetic mapping of plasmid p4093 using restriction enzyme analysis.
  • Identification of gene clusters associated with resistance and conjugation.

Main Results:

  • Three of five isolated antibiotic-resistant plasmids were found to be conjugative.
  • Plasmid p4093 (51.5 kb) was mapped, revealing a cluster of restriction sites associated with streptomycin and tetracycline resistance.
  • Genes conferring conjugative ability were located in at least two distinct regions of p4093.

Conclusions:

  • Conjugative antibiotic-resistant plasmids are present in virulent Bordetella avium strains.
  • The genetic organization of p4093 suggests specific regions are responsible for resistance and transfer.
  • Further research into these plasmids can inform strategies to combat antibiotic resistance in B. avium infections.

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