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Enhanced levels of multicatalytic proteinase mRNAs in Rous sarcoma virus transformed cells
D F Balson1, H E Skilton, S T Sweeney
1Department of Biochemistry, University of Leicester, U.K.
Biological Chemistry Hoppe-Seyler
|July 1, 1992
Abstract:
The multicatalytic proteinase (proteasome; MCP) is a high molecular mass proteinase which is found in all eukaryotic cells. Northern blot analysis of the levels of MCP mRNAs in a Rat-1 fibroblast cell line and in cells transformed with Rous sarcoma virus showed marked increases in the transformed cells. However, the results of immunoblot analysis with anti-MCP antibodies suggested that the MCP protein content of the two cell lines was similar.