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Related Experiment Videos

MKW, a novel hematopoietic antigen.

M Koehler1, T J Fitzgerald, R M Goorha

  • 1Department of Hematology-Oncology, St Jude Children's Research Hospital, Memphis, Tennessee.

Leukemia
|October 11, 1992
PubMed
Summary

A novel hematopoietic antigen, MKW, was identified on monocytic and myelocytic cells, with early expression in normal hematopoiesis. This antigen shows prognostic significance in childhood leukemia.

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Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • A novel hematopoietic antigen, termed MKW, was identified using a murine monoclonal antibody.
  • MKW was detected on specific cell lines (KG-1, U937) but not others (K562, ML1, HL-60).

Purpose of the Study:

  • To characterize the novel hematopoietic antigen MKW.
  • To investigate the expression pattern of MKW on normal and malignant hematopoietic cells.
  • To determine the biochemical properties and potential role of MKW in hematopoiesis and leukemia.

Main Methods:

  • Monoclonal antibody production and characterization.
  • Flow cytometry for antigen detection on cell lines and primary cells.
  • Immunoprecipitation and biochemical assays (e.g., phosphatidylinositol-phospholipase C treatment) for protein characterization.

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Main Results:

  • MKW is expressed on monocytic, myelocytic cells, and a B-cell subpopulation in normal hematopoiesis, with highest early expression on monocytic cells.
  • Surface expression of MKW is upregulated during U937 cell differentiation but not by T- or B-cell activation.
  • MKW is a 52-kDa nonglycosylated protein, not attached to a glycolipid anchor, and is also found intracellularly in some hematopoietic cells.
  • MKW was detected on leukemic blasts in a significant percentage of children with acute myeloblastic leukemia (27%) and acute lymphoblastic leukemia (13%).

Conclusions:

  • MKW is a novel hematopoietic antigen with a distinct expression pattern and biochemical characteristics.
  • MKW expression correlates with cell differentiation and has prognostic significance in childhood leukemia.
  • Further investigation into the role of MKW in hematopoiesis and its potential as a diagnostic or therapeutic target is warranted.