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Updated: Feb 9, 2026

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
Published on: April 30, 2018
Effects of calcium on Na+,K(+)-ATPase isolated from human placenta
L Mazzanti1, R A Rabini, A M Cugini
1Istituto di Biochimica, Facoltà di Medicina e Chirurgia, Università degli Studi, Ancona, Italy.
Abstract:
It has been reported that Ca2+ can be either stimulatory or inhibitory of the activity of Na+,K(+)-ATPase obtained from different sources. The aim of the present work was to study the effect of increasing concentrations of Ca2+ on Na+,K(+)-ATPase activity and of placenta. The temperature dependence of Na+,K(+)-ATPase activity and of spectral parameters of a spin label paramagnetic maleimide) were also (MSL, 2,2,6,6-tetramethylpiperidin-1-oxyl-4-maleimide) were also investigated in absence and in presence of 2 mM Ca2+. Ca2+ affects positively the enzymatic activity and this effect is evident at all temperature tested. Maleimide spectral parameters are not affected by the presence of 2 mM Ca2+, showing that the microenvironments of SH groups are not modified following ion interactions.
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