Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Na/H antiporter].

K Takaichi1

  • 1Fourth Department of Internal Medicine, University of Tokyo.

Nihon Rinsho. Japanese Journal of Clinical Medicine
|September 1, 1992
PubMed
Summary
This summary is machine-generated.

Two sodium-hydrogen antiporter (Na/H) isoforms maintain cell pH, differing in amiloride sensitivity and cellular location. Research is ongoing to understand their distinct roles and functions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Helicobacter cinaedi bacteremia in four renal transplant patients: clinical features and an important suggestion regarding the route of infection.

Transplant infectious disease : an official journal of the Transplantation Society·2015
Same author

Once-weekly teriparatide in hemodialysis patients with hypoparathyroidism and low bone mass: a prospective study.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2015
Same author

Colovesical Fistula After Renal Transplantation: Case Report.

Transplantation proceedings·2015
Same author

Cyst infection in autosomal dominant polycystic kidney disease: causative microorganisms and susceptibility to lipid-soluble antibiotics.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2015
Same author

Bone histomorphometry in a long-term hemodialysis patient with hypoparathyroidism and sarcoidosis.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2014
Same author

Impact of tubulointerstitial lesions on anaemia in patients with biopsy-proven diabetic nephropathy.

Diabetic medicine : a journal of the British Diabetic Association·2014

Area of Science:

  • Cellular Biology
  • Biochemistry
  • Physiology

Background:

  • The plasma membrane's sodium-hydrogen antiporter (Na/H) is crucial for cellular pH homeostasis.
  • Two isoforms, differing in amiloride sensitivity, have been identified, suggesting distinct genetic origins.
  • These isoforms exhibit differential localization within epithelial and non-epithelial cells.

Purpose of the Study:

  • To elucidate the distinct roles and characteristics of the amiloride-sensitive and amiloride-resistant Na/H antiporter isoforms.
  • To investigate the molecular basis and functional significance of these antiporter variants.

Main Methods:

  • Identification and characterization of Na/H antiporter isoforms.
  • Analysis of differential cellular localization (apical vs. basal membranes).

Related Experiment Videos

  • Cloning and sequencing of the amiloride-sensitive Na/H antiporter.

    • Main Results:

    • The amiloride-sensitive Na/H antiporter, found in non-epithelial and basal epithelial cells, has been cloned and sequenced.
    • This isoform is a glycoprotein comprising 815 amino acids.
    • The amiloride-resistant form is located in the apical membrane of epithelial cells and is vital for bicarbonate transport.

    Conclusions:

    • Distinct Na/H antiporter isoforms play specialized roles in cellular pH regulation and vectorial transport.
    • Further functional analysis using mutant cDNA is underway to fully characterize Na/H antiporter mechanisms.