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Transposon mutagenesis in Legionella pneumophila. I.--Persistence of suicide and broad host-range plasmids
M Tully1, S A Sharpe, S Ashworth
1Division of Pathology, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, UK.
Abstract:
Two of three highly virulent strains of Legionella pneumophila could act as recipients at high frequencies in conjugation experiments with Escherichia coli donor strains carrying broad host-range plasmids belonging to incompatibility groups N, P and W. All broad host-range and most transposon-delivery plasmids persisted within transconjugants with high stability. Only one (pSUP1021) of several vehicles designed for the delivery of transposons into the chromosome of Gram-negative bacteria was found to yield transposon mutants of Legionella at a detectable frequency.
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