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Miniature urea sensor based on H(+)-ion sensitive field effect transistor and its application in clinical analysis
Chinese Journal of Biotechnology
|January 1, 1992
Summary
This study developed a novel urea-Enzyme-Field-Effect Transistor (ENFET) sensor for rapid and accurate urea and Blood Urea Nitrogen (BUN) detection. The developed sensor demonstrates high sensitivity, stability, and reliability for clinical applications.
Area of Science:
- Biomedical Engineering
- Chemical Sensors
- Biosensors
Background:
- Enzyme field-effect transistors (ENFETs) offer a promising platform for biosensing.
- Accurate and rapid detection of urea is crucial for diagnosing kidney function and other medical conditions.
Purpose of the Study:
- To develop and characterize a novel urea-ENFET probe for sensitive and stable urea detection.
- To evaluate the performance of the urea-ENFET for Blood Urea Nitrogen (BUN) determination in biological samples.
Main Methods:
- Fabrication of a dual ISFET probe with BSA-urease and BSA membranes.
- Differential measurements to assess sensor response characteristics.
- Calibration using varying urea concentrations in phosphate buffer.
- Comparison with established enzymatic methods for BUN analysis.
Main Results:
- The urea-ENFET exhibited rapid response times (10-60s) across different urea concentrations.
- Linear detection ranges of 1.0-8.0 mg/dl (R=0.997) and 0.1-1.0 mg/dl (R=0.998) were achieved.
- High correlation (r=0.9912) was observed when comparing BUN values with enzymatic methods.
- The sensor maintained over 90% response sensitivity after 250 runs over 1.5 months.
Conclusions:
- The developed urea-ENFET probe is a sensitive, stable, and reliable biosensor for urea and BUN determination.
- Its performance indicates significant potential for practical clinical diagnostics.
- The sensor's long-term stability and accuracy make it suitable for routine laboratory use.