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Published on: January 27, 2014
Characterization of H2 histamine receptor: linkage to both adenylate cyclase and [Ca2+]i signaling systems
1Department of Internal Medicine, University of Michigan and Medical Center, Ann Arbor 48109.
Abstract:
We undertook these studies to examine the intracellular mechanisms of histamine action using a rat hepatoma-derived cell line that had been transfected to express the H2 histamine receptor cDNA. Transfected cells demonstrated increased adenosine 3',5'-cyclic monophosphate production, membrane inositol phospholipid turnover, and intracellular Ca2+ concentration ([Ca2+]i) in response to histamine. All of the effects could be inhibited with the H2 histamine receptor antagonist cimetidine, and the increased membrane inositol phospholipid turnover and [Ca2+]i were abolished by cholera toxin pretreatment of cells. These data support the notion that a single histamine H2 receptor can be linked to two stimulatory intracellular signaling systems.
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