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Cleavage and deprotection of peptides on MBHA-resin with hydrogen bromide
S S Wang1, B S Wang, J L Hughes
1Alpha 1 Biomedicals, Inc., Foster City, CA.
International Journal of Peptide and Protein Research
|September 1, 1992
Abstract:
Dilute hydrogen bromide in trifluoroacetic acid containing pentamethylbenzene and thioanisole was used in the cleavage and deprotection of peptides on MBHA-resin. Particular attention was paid to potential applicability of the method to kilogram scale synthesis of thymosin alpha 1. In the HPLC purification of the peptides, acetonitrile was replaced by relatively nontoxic isopropanol. The change should be economically and environmentally very attractive.