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Enzymatic synthesis of PAPS with an ATP-regeneration system
H Ibuki1, T Tashiro, M Hayashi
1Biochemistry Department, Unitika Research and Development Center, Kyoto, Japan.
Nucleic Acids Symposium Series
|January 1, 1992
Abstract:
Sulfate activating enzymes, ATP sulfurylase and APS kinase, were newly isolated from a thermophile, Bacillus stearothermophilus. Adenosine 3'-phosphate 5'-phosphosulfate (PAPS) was synthesized by these enzymes. The reaction proceeded more efficiently when an ATP-regeneration system, using acetate kinase, was coupled to the reaction system.