Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence-specific DNA recognition by basic peptides.

T Morii1, M Shimomura, I Saito

  • 1Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Japan.

Nucleic Acids Symposium Series
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

All-trans-retinoic acid inhibits tumour growth of malignant pleural mesothelioma in mice.

The European respiratory journal·2009
Same author

Characterization of monoclonal antibodies directed against squamous cell carcinoma antigens: report of the second TD-10 workshop.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine·2009
Same author

[Risk management for congenital heart surgery].

Kyobu geka. The Japanese journal of thoracic surgery·2008
Same author

NOTES transvaginal video-assisted cholecystectomy: first series.

Endoscopy·2008
Same author

Depositional records of plutonium and (137)Cs released from Nagasaki atomic bomb in sediment of Nishiyama reservoir at Nagasaki.

Journal of environmental radioactivity·2008
Same author

Observation of time-dependent CP violation in B0 --> eta'K0 decays and improved measurements of CP asymmetries in B0 --> phiK0, KS0KS0KS0 and B0 --> J/psiK0 decays.

Physical review letters·2007
Same journal

Nucleotide specificity of DNA cleavage by esperamycin/calichemicin antitumor antibiotics.

Nucleic acids symposium series·2018
Same journal

A model reaction for the repair of nucleic acids. The repair of thymidine bromohydrins.

Nucleic acids symposium series·2018
Same journal

Effects of base damages on DNA replication.

Nucleic acids symposium series·2018
Same journal

Effect of serine residue on the effectiveness of cationic polypeptide-based gene delivery.

Nucleic acids symposium series·2003
Same journal

Smart probe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator.

Nucleic acids symposium series·2003
Same journal

The engineering, structure, and DNA binding properties of a novel His4-type zinc finger peptide.

Nucleic acids symposium series·2003
See all related articles

A chiral template modeled a dimeric interface for DNA binding proteins. Only one specific dimer arrangement effectively bound DNA with the native MyoD binding sequence.

Area of Science:

  • Molecular biology
  • Structural biology
  • Biochemistry

Background:

  • DNA binding proteins are crucial for gene regulation.
  • The MyoD protein, a helix-loop-helix class DNA binding protein, dimerizes to bind DNA.
  • Understanding dimeric interfaces is key to deciphering protein-DNA interactions.

Purpose of the Study:

  • To model the dimeric interface of a DNA binding protein.
  • To investigate the role of chirality and subunit arrangement in DNA binding.
  • To identify the specific dimeric configuration of MyoD that binds its target DNA sequence.

Main Methods:

  • Utilized a chiral template with C2 symmetry for modeling.
  • Tethered an oligopeptide derived from the MyoD basic region to the template.

Related Experiment Videos

  • Synthesized and analyzed four distinct dimer models varying in chirality and polarity.

    • Main Results:

    • Only one of the four modeled dimer configurations demonstrated DNA binding capability.
    • The effective dimer model featured a right-handed chirality and a C-terminus to C-terminus subunit arrangement.
    • This specific model successfully bound DNA containing the native MyoD binding sequence.

    Conclusions:

    • The chirality and specific C-terminus to C-terminus arrangement of MyoD dimer subunits are critical for DNA binding.
    • Chiral templates provide a viable method for modeling protein dimeric interfaces.
    • This study elucidates key structural requirements for MyoD-DNA recognition.