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Axenic massive cultivation of Entamoeba histolytica trophozoites in spinner flasks and in a microfermentor
S Said-Fernández1, B D Mata-Cárdenas, J Castro-Garza
1División de Biología Celular, Instituto Mexicano del Seguro Social, Monterrey, NL.
Archives of Medical Research
|January 1, 1992
Abstract:
A medium, PEHPS, whose main components are extracts of ox liver, and ox and swine pancreas (EHP) was used to develop two alternative methods for axenic cultivation of trophozoites in suspension: with magnetic stirrer and with a 14 l capacity microfermentor, giving the first technic yields of 2.36 x 10(5) to 3.08 x 10(5) and the second in a 14 l capacity microfermentor, which produced 1.65 x 10(5) amebas/ml in one batch and 2.94 to 3.65 x 10(5) of such parasites/ml at semicontinuous flux.