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Updated: Aug 18, 2026

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
Published on: September 6, 2018
Prolactin-like substance secretion by granulosa cells isolated from bovine ovaries
A V Sirotkin1, J Nitray, V P Politov
1Research Institute of Animal Production, Nitra, Slovakia.
Abstract:
By the 5-day culture of bovine granulosa cells both in serum-free and in serum-supplemented medium the time-dependent accumulation of PRL immunoreactivity was observed. FSH additions (10-10,000 ng/ml medium) led to a dramatic rise of immunoreactive PRL in a dose-dependent manner. LH stimulated the increase of PRL-like substance only in a great dose (10 IU/ml). Lower LH doses (0.01-1 IU/ml) had no significant influence on this process. Low doses of oxytocin (1 or 10 mIU/ml) blocked, and higher ones (100 or 1,000 mIU/ml) stimulated the granulosa PRL-like substance accumulation. Arginine-8-vasopressin (1-1,000 ng/ml), arginine-8-vasotocin (10-10,000 ng/ml), or LH-RH (10-10,000 ng/ml) failed to influence the PRL immunoreactivity accumulation in the culture medium. Present data may suggest the production of PRL-like substance by bovine ovarian cells, as well as the involvement of gonadotropins and oxytocin in the regulation of this process.
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