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[An immunoenzyme test (ELISA) for the detection of circulating class-IgA antibodies in human leptospirosis]

M V da Silva1, E D Camargo

  • 1Seçáo de Sorologia, Instituto Adolfo Lutz, Secretaria de Saúde do Estado de Sáo Paulo, Brasil.

Revista Do Instituto De Medicina Tropical De Sao Paulo
|May 1, 1992
PubMed

An enzyme-linked immunosorbent assay ELISA was evaluated for the detection of IgA antibodies in the human leptospirosis. The assay proved to be sensitive and specific when compared with the ELISA-IgM, in the examined serum samples. The results found suggest that IgA antibodies became positive later in leptospirosis, and will can be an evolutive indicator in the development of the disease.

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.