Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Crocodilian evolution: insights from immunological data.

C A Hass1, M A Hoffman, L D Densmore

  • 1Department of Biology, Pennsylvania State University, University Park 16802.

Molecular Phylogenetics and Evolution
|September 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomarkers of Recent Cannabis Use in Blood, Oral Fluid and Breath.

Journal of analytical toxicology·2021
Same author

The Envirome Web Service: Patient context at the point of care.

Journal of biomedical informatics·2021
Same author

Presynaptic inhibition decreases when estrogen level rises.

Scandinavian journal of medicine & science in sports·2018
Same author

Visualization of molecular structures using HoloLens-based augmented reality.

AMIA Joint Summits on Translational Science proceedings. AMIA Joint Summits on Translational Science·2017
Same author

TERTIARY SPECIATION MODELS IN AUSTRALIAN ANURANS: MOLECULAR DATA CHALLENGE PLEISTOCENE SCENARIO.

Evolution; international journal of organic evolution·2017
Same author

Spinal and supraspinal motor control predictors of rate of torque development.

Scandinavian journal of medicine & science in sports·2014

Microcomplement fixation analysis of serum albumin evolution in Crocodylia reveals phylogenetic relationships. Molecular data challenges traditional placements, suggesting reevaluation of morphological evidence for accurate reptile evolution understanding.

Area of Science:

  • Evolutionary Biology
  • Molecular Phylogenetics
  • Immunology

Background:

  • Understanding the evolutionary history and phylogenetic relationships within the order Crocodylia is crucial for reptile classification.
  • Previous studies have utilized immunodiffusion techniques to assess albumin evolution, providing a basis for further investigation.

Purpose of the Study:

  • To investigate the evolutionary relationships among Crocodylia using the quantitative microcomplement fixation technique on serum albumin.
  • To compare the efficacy of microcomplement fixation with immunodiffusion for assessing albumin sequence divergence and phylogenetic inference.
  • To reconcile molecular data with existing fossil records and morphological classifications, particularly regarding the placement of Gavialis.

Main Methods:

  • Employed microcomplement fixation, a quantitative immunological assay, to analyze serum albumin evolution in Crocodylia.

Related Experiment Videos

  • Utilized previously established albumin antisera and antigens, whose cross-reactivity was confirmed by qualitative immunodiffusion.
  • Compared divergence times predicted by the albumin molecular clock with estimates from the fossil record.

    • Main Results:

    • Microcomplement fixation provided more discriminating data on albumin sequence divergence compared to immunodiffusion.
    • Phylogenetic conclusions from both methods were congruent, supporting the families Alligatoridae and Crocodylidae, and placing Gavialis as sister to Tomistoma.
    • Molecular data consistently contradicted the traditional placement of Gavialis outside the extant crocodilians.

    Conclusions:

    • Microcomplement fixation is a powerful tool for resolving phylogenetic relationships based on albumin evolution in Crocodylia.
    • The molecular evidence strongly suggests that the traditional phylogenetic placement of Gavialis requires reevaluation.
    • A comprehensive reexamination of both extant and fossil morphological data is warranted to align with molecular findings in crocodilian phylogeny.