[The lysosomal system of the hepatocytes during the development of extrahepatic cholestasis (a quantitative...

Electron microscopic and morphometric studies of the lysosomal system of hepatocytes with quantitative, informative and dispersion analysis of electron micrographs in healthy rats and rats with extrahepatic cholestasis have revealed the stage-like character of changes of the reaction of the liver subcellular structures in the processes of development of the pathologic process. At the first stage (12h-3 days) in parallel with the symptoms of injury (dilation of the lumen of bile canals with dystrophic alterations of epitheliocytes microvilli, depletion of the cytoplasm by glycogen, appearance of lipid inclusions, accumulation of the bile pigments) the initiation of urgent reactions of compensation was observed (using part of primary lysosomes in heterophagic processes, activation of autophagocytosis) as well as the beginning of the formation of long-term reactions with a somewhat increased amount of primary lysosomes and greater amount of small primary forms. There occur normalization of the ratio of primary and secondary forms. The 2nd stage (the 2nd half of the 1st week--the 2nd week) is characterized by the prevalence of compensatory adaptation reactions, underlaid by the formation of long-term reactions of compensation (considerable increase of the amount of primary lysosomes at the expense of intensification of their synthesis, active resorption of bile and lipid metabolites with the transformation of the latter in glycogen). The 3rd stage (the 3rd week) is characterized by depletion of reserves of the lysosomal system: sharp drop of the ability for self-renewal of the structures, change of the population composition of organelles expressed as prevalence of autophagosomes and residual bodies, transformation of the lysosomes in residual bodies.