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Structural changes induced by He-Ne laser on the chick embryo ovary
1II Cátedra de Histología, Embriología y Genética, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, República Argentina.
Revista De La Facultad De Ciencias Medicas (Cordoba, Argentina)
|January 1, 1992
Summary
He-Ne laser irradiation of chicken ovaries at 7 days of incubation caused tissue disorganization and reduced mucosubstances. Lymphocytic infiltration was observed, indicating a potential inflammatory response to laser exposure in developing gonads.
Area of Science:
- Developmental biology
- Histology
- Biophysics
Background:
- The chicken ovary's development involves specific cellular and extracellular matrix components.
- Understanding the impact of external stimuli on embryonic gonadal development is crucial.
Purpose of the Study:
- To investigate the morpho-histochemical effects of Helium-Neon (He-Ne) laser irradiation on chicken ovaries at 7 days of incubation.
- To assess changes in tissue structure and histochemical properties following laser exposure.
Main Methods:
- Chicken embryos at 7 days of incubation were irradiated with a 5 mW He-Ne laser (632.8 nm) for 5 minutes.
- Ovaries were processed using Hematoxylin/Eosin (H/E), Periodic Acid-Schiff (PAS), Alcian blue, and Toluidine blue staining techniques.
- Histological and histochemical analyses were performed on the irradiated gonads.
Main Results:
- Irradiated ovaries exhibited significant tissue disorganization compared to controls.
- Histochemical reactions for mucosubstances (PAS and Alcian blue positive material) were largely negative in irradiated tissues.
- A notable lymphocytic infiltration was observed in the stroma of the irradiated gonads.
Conclusions:
- He-Ne laser irradiation at the studied parameters decreases ovarian mucosubstances, particularly those associated with cell membranes and basement membranes.
- Laser exposure induces an inflammatory response characterized by mononuclear cell infiltration in developing chicken ovaries.
- Germ and epithelial cells remained structurally unaltered, suggesting differential sensitivity within the gonad.