[Experiences with DNCB sensitization in normal human individuals of various age groups (author's transl)]

Using DNCB contact sensitization, the primary cellular immune response of 40 normal individuals was investigated. The response to DNCB in the younger age group was higher and stronger than in the older age group, suggesting a decrease of T-cell function in elderly subjects. The simultaneous application of a high (1000 mug) and a low (100 mug) dose of DNCB for quantitation of the reactivity gave good results. However, in several cases - especially in the younger age group - the local reactions reached unexpected intensity followed by long lasting pigmentation. One investigator exhibited contact eczema on both hands after sensitization by merely touching the skin area of a previous DNCB reaction. Because of the potent immunogenicity of this substance and the possibility of cross reactions with numerous similar antigens widely used in the chemical and related industry, this test should only be applied in selected cases.