Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enkephalin hydrolysis by mouse plasma in vitro.

S Shibanoki1, S B Weinberger, G Schulteis

  • 1Department of Pharmacology, Nihon University School of Medicine, Tokyo, Japan.

Life Sciences
|January 11, 1992
PubMed
Summary

Mouse plasma rapidly breaks down Met-enkephalin compared to Leu-enkephalin. This hydrolysis involves specific enzymes like aminopeptidase M, enkephalinase, and angiotensin-converting enzyme, influencing peptide stability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ψ(2S) Suppression in Pb-Pb Collisions at the LHC.

Physical review letters·2024
Same author

Measurements of Groomed-Jet Substructure of Charm Jets Tagged by D^{0} Mesons in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2023
Same author

Measurement of the Lifetime and Λ Separation Energy of _{Λ}^{3}H.

Physical review letters·2023
Same author

Measurement of the J/ψ Polarization with Respect to the Event Plane in Pb-Pb Collisions at the LHC.

Physical review letters·2023
Same author

Enhanced Deuteron Coalescence Probability in Jets.

Physical review letters·2023
Same author

First Measurement of Antideuteron Number Fluctuations at Energies Available at the Large Hadron Collider.

Physical review letters·2023

Area of Science:

  • Biochemistry
  • Pharmacology
  • Neuroscience

Background:

  • Enkephalins are endogenous opioid peptides involved in pain modulation and other physiological processes.
  • Understanding the degradation pathways of enkephalins is crucial for developing therapeutic strategies.
  • Mouse plasma serves as a relevant model for studying peptide metabolism in vivo.

Purpose of the Study:

  • To investigate and compare the hydrolysis rates of [Leu]enkephalin and [Met]enkephalin in mouse plasma.
  • To identify the specific peptidases responsible for enkephalin degradation in plasma.
  • To determine the half-lives of [Leu]enkephalin and [Met]enkephalin in mouse plasma.

Main Methods:

  • High-Performance Liquid Chromatography with Electrochemical Detection (HPLC-ECD) was employed to quantify Tyr-containing metabolites.

Related Experiment Videos

  • In vitro hydrolysis assays were performed using pooled whole mouse plasma.
  • Selective peptidase inhibitors were used to identify enzyme involvement in the hydrolysis process.

    • Main Results:

    • Accumulation of Tyr-Gly-Gly was significantly higher from [Met]enkephalin hydrolysis compared to [Leu]enkephalin.
    • [Met]enkephalin exhibited a shorter half-life in mouse plasma (approximately half) than [Leu]enkephalin.
    • Enkephalinase, angiotensin-converting enzyme, and a bestatin-sensitive aminopeptidase (likely aminopeptidase M) were identified as key enzymes in plasma hydrolysis.

    Conclusions:

    • Mouse plasma efficiently metabolizes [Met]enkephalin, with a faster degradation rate than [Leu]enkephalin.
    • Multiple peptidases, including aminopeptidase M, enkephalinase, and ACE, contribute to the in vitro hydrolysis of enkephalins in mouse plasma.
    • These findings provide insights into the pharmacokinetic profile and enzymatic inactivation of enkephalins.