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Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

Early changes at the epiphysis of rachitic chicks, following administration of vitamin D

L F BELANGER, B B MIGICOVSKY

    The Journal of Experimental Medicine
    |June 1, 1958
    PubMed

    Insights

    Vitamin D supplementation rapidly restored bone mineralization and cartilage maturation in vitamin D-deficient chicks. This study highlights vitamin D

    Area of Science:

    • Biochemistry
    • Histology
    • Mineral Metabolism

    Background:

    • Vitamin D deficiency in young chicks leads to rickets.
    • Rickets is characterized by impaired bone mineralization and cartilage abnormalities.

    Purpose of the Study:

    • To investigate the effects of vitamin D repletion on bone healing in vitamin D-deficient chicks.
    • To examine the histological and biochemical changes during recovery from rickets.

    Main Methods:

    • Chicks were fed a vitamin D-deficient diet, then supplemented orally with vitamin D.
    • Tibiae were analyzed using radiography, histological staining (P.A.S.), ashing, and Ca(45) autoradiography.

    Main Results:

    • Mineralization and new bone spicule formation reappeared within 2 days of vitamin D treatment.
    • Immature cartilage rapidly matured morphologically.
    • The rachitic matrix, rich in cation binders like chondroitin sulfate, showed progressive loss of these components.

    Conclusions:

    • Vitamin D is crucial for rapid restoration of bone mineralization and cartilage development.
    • Chondroitin sulfate may play a role in the rachitic matrix and its turnover during healing.

    Keywords:
    EPIPHYSES/effect of drugs onRICKETS/experimentalVITAMIN D/effects

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