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Induction and function of eosinophil intercellular adhesion molecule-1 and HLA-DR
T T Hansel1, I J De Vries, J M Carballido
1Swiss Institute of Allergy and Asthma Research (SIAF), Davos-Platz.
Journal of Immunology (Baltimore, Md. : 1950)
|September 15, 1992
Summary
Tissue eosinophils, unlike blood eosinophils, express ICAM-1 and HLA-DR. Cytokines and TNF induce these molecules, enabling eosinophils to interact with T cells and present antigens, suggesting a role in immune responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- Eosinophils in asthmatic sputum express ICAM-1 and HLA-DR, but peripheral blood eosinophils do not.
- The induction of these molecules on eosinophils is crucial for understanding their role in inflammation and immune responses.
Purpose of the Study:
- To investigate the mechanisms of ICAM-1 and HLA-DR induction on human eosinophils.
- To determine the functional consequences of ICAM-1 and HLA-DR expression on eosinophils.
Main Methods:
- Purified human blood eosinophils were cultured with T cell supernatants and recombinant cytokines.
- Flow cytometry was used to assess ICAM-1 and HLA-DR expression.
- Functional assays measured eosinophil adhesion to T cells and antigen-specific T cell proliferation.
Main Results:
- ICAM-1 and HLA-DR were induced on eosinophils by T cell supernatants, with maximal induction at 48 hours.
- Synergy between eosinophil survival factors (IL-5, IL-3, GM-CSF) and TNF induced ICAM-1.
- Synergy between IL-3 and IFN-gamma induced HLA-DR.
- ICAM-1 expression increased eosinophil adhesion to T cells.
- HLA-DR expression enabled eosinophils to mediate antigen-specific T cell proliferation.
Conclusions:
- Eosinophils can be induced to express ICAM-1 and HLA-DR through cytokine signaling.
- Induced ICAM-1 and HLA-DR confer functional capabilities on eosinophils, including enhanced adhesion to T cells and antigen presentation.
- These findings suggest that tissue eosinophils play an active role in leukocyte interactions and antigen presentation within inflammatory sites.