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12 alpha-hydroxytestosterone. A hitherto unidentified testosterone metabolite produced by cytochrome P-450 2A2
S J Smith1, K R Korzekwa, T Aoyama
1Laboratory of Chemical Pharmacology, National Heart, Lung & Blood Institute, National Institutes of Health, Bethesda, MD 20892.
Abstract:
Several cytochrome P-450 enzymes are able to hydroxylate testosterone to many different metabolites, some of which remain to be identified. One hitherto unidentified metabolite represents a major metabolite of P-450 2A2, present in the liver of adult male rats, and a minor metabolite of several other cytochrome P-450 enzymes. Using the techniques of HPLC and GC/MS, we have obtained unequivocal evidence that this metabolite is 12 alpha-hydroxytestosterone.