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Adherence of Staphylococci to plastic, mesothelial cells and mesothelial extracellular matrix

M G Betjes1, C W Tuk, D G Struijk

  • 1Department of Cell Biology, Vrije Universiteit, Amsterdam, The Netherlands.

Advances in Peritoneal Dialysis. Conference on Peritoneal Dialysis
|January 1, 1992
PubMed

Insights

Staphylococci adherence to mesothelial cells and their extracellular matrix was investigated. Results suggest no increased risk for Staphylococci adherence when mesothelial extracellular matrix is exposed.

Area of Science:

  • Microbiology
  • Cell Biology
  • Biomaterials

Background:

  • Mesothelial cells (MC) and their extracellular matrix (ECM) are crucial in peritoneal dialysis (PD).
  • Understanding bacterial adherence to these surfaces is vital for preventing PD-related infections.

Purpose of the Study:

  • To evaluate mesothelial cells (MC) and mesothelial extracellular matrix (ECM) as substrates for Staphylococci adherence.
  • To compare bacterial adherence to MC and ECM with adherence to plastic.

Main Methods:

  • Mesothelial cells (MC) isolated from peritoneal dialysis effluent.
  • MC monolayers characterized for extracellular matrix (ECM) presence using ELISA, detecting fibronectin and laminin.
  • Bacterial adherence assays performed with Staphylococcus aureus and Staphylococcus epidermidis isolates.

Main Results:

  • Staphylococcus aureus adhered significantly better to both MC monolayers (40.2%) and ECM (33.4%) compared to plastic.
  • Staphylococcus epidermidis showed similar adherence to plastic (22.1%) and MC monolayers (23.5%).
  • Mesothelial ECM was a poor substrate for Staphylococcus epidermidis adherence (6.8%).

Conclusions:

  • Mesothelial cells and their ECM support Staphylococcus aureus adherence.
  • Mesothelial ECM does not appear to increase the risk of Staphylococci adherence in peritoneal dialysis.
  • Further research is needed to fully elucidate the role of mesothelial surfaces in Staphylococci infections.

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