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Oxygen-induced changes in pulmonary superoxide dismutase assayed by antibody titrations
The American Journal of Physiology
|October 1, 1976
Summary
Superoxide dismutase (SOD) activity increases in rat lungs adapting to oxygen. This study shows cuprozinc SOD accounts for most of the observed increase, suggesting its key role in oxygen tolerance.
Area of Science:
- Biochemistry
- Physiology
- Toxicology
Background:
- Pulmonary superoxide dismutase (SOD) activity increases with oxygen adaptation.
- SOD is suggested to play a crucial role in developing oxygen tolerance.
Purpose of the Study:
- To investigate the specific role of cuprozinc SOD in oxygen tolerance.
- To quantify the contribution of cuprozinc SOD to increased pulmonary SOD activity.
Main Methods:
- Purification of cuprozinc SOD from rat liver.
- Production of rabbit antisera against rat cuprozinc SOD.
- Antibody titration to measure SOD levels in rat lungs exposed to 85% oxygen.
Main Results:
- Cuprozinc SOD was purified and characterized.
- Antibody titration revealed a 41% increase in absolute cuprozinc SOD.
- This increase accounted for most of the 48% rise in total pulmonary SOD activity.
- Evidence suggests the presence of manganese SOD, but its role remains unclear.
Conclusions:
- Cuprozinc SOD is the primary contributor to the increased pulmonary SOD activity observed in oxygen-adapted rats.
- These findings support the significant role of cuprozinc SOD in the development of oxygen tolerance.