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Polymorphism of the Pa gene
A G Vishteh1, H W Kunz, A L Cortese Hassett
1Department of Pathology, University of Pittsburgh, School of Medicine, Pennsylvania 15261.
American Journal of Reproductive Immunology (New York, N.Y. : 1989)
|September 1, 1992
Summary
Researchers identified three alleles of the Pa gene using DNA analysis. These alleles correlate with varying levels of anti-Pa antibody response in rat strains, impacting immune reactivity.
Area of Science:
- Immunogenetics
- Molecular Biology
Background:
- The Pa gene is found in rat strains, with distinct DNA-level variations.
- Restriction Fragment Length Polymorphisms (RFLP) differentiate Pa+ and Pa- strains.
Purpose of the Study:
- To determine the number of Pa gene alleles at the DNA level.
- To correlate Pa gene alleles with anti-Pa antibody response in rat strains.
Main Methods:
- DNA analysis using restriction enzymes and probes.
- Examination of RFLP patterns in various rat strains.
- Correlation of RFLP with anti-Pa antibody reactivity.
Main Results:
- Distinct RFLP patterns were observed for Pa+ and Pa- strains.
- Rat strains with the 'b' haplotype showed an intermediate RFLP pattern.
- These 'b' haplotype strains exhibited low reactivity with anti-Pa antibody.
Conclusions:
- Three alleles of the Pa gene exist, categorized by expression level: Paa (high), Pab (low), and Pa- (none).
- Allelic variations influence the immune response and antibody production.