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Related Experiment Videos

Continuous recombinant bacterial fermentations utilizing selective flocculation and recycle.

K L Henry1, R H Davis, A L Taylor

  • 1Department of Chemical Engineering, University of Colorado, Boulder 80309-0424.

Biotechnology Progress
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

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Selective recycle successfully maintained unstable plasmid-bearing bacteria in continuous reactors. This method uses differential sedimentation to separate and recycle desired cells, preventing culture reversion.

Area of Science:

  • Biotechnology
  • Microbial Engineering
  • Chemical Engineering

Background:

  • Maintaining unstable plasmid-bearing bacterial strains in continuous culture is challenging due to faster-growing segregant cells.
  • Plasmid loss leads to segregant cells with a metabolic advantage (faster growth) and altered physical properties (nonflocculent).

Purpose of the Study:

  • To demonstrate the effectiveness of selective recycle in maintaining a dominant, unstable plasmid-bearing bacterial strain in a continuous reactor.
  • To validate the selective recycle reactor theory and present a predictive criterion for successful strain maintenance.

Main Methods:

  • Utilizing a continuous reactor (chemostat) coupled with an inclined settler for cell separation.
  • Employing differential sedimentation to separate flocculent, plasmid-bearing cells from nonflocculent segregant cells.

Related Experiment Videos

  • Recycling the underflow enriched with plasmid-bearing cells back to the chemostat.

    • Main Results:

    • Selective recycle successfully maintained the plasmid-bearing strain as dominant in the continuous reactor.
    • Without selective recycle, the culture reverted to 100% segregant cells.
    • Experimental results aligned with selective recycle reactor theory.

    Conclusions:

    • Selective recycle is an effective strategy for maintaining unstable plasmid-bearing bacterial strains in continuous culture.
    • A predictive criterion based on selective recycle reactor theory can determine the feasibility of maintaining such strains.