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Environmental effects on protein glycosylation.
1Department of Chemical Engineering, Stanford University, CA 94305-5025.
Bio/Technology (Nature Publishing Company)
|May 1, 1990
Summary
Mammalian cell cultures are key for producing therapeutic proteins. This review highlights how the cell culture environment directly impacts protein glycosylation, a critical modification affecting biological activity.
Area of Science:
- Biotechnology
- Biochemistry
- Cell Biology
Background:
- Mammalian cells are utilized for producing therapeutic and diagnostic proteins.
- These cells perform crucial post-translational modifications like glycosylation.
- Glycosylation significantly influences glycoprotein properties such as clearance, immunogenicity, and activity.
Purpose of the Study:
- To review and organize existing data on the influence of the cell culture environment on N-linked glycosylation.
- To consolidate findings from in vitro cell and tissue culture studies.
Main Methods:
- Literature review of in vitro cell and tissue culture studies.
- Organization and synthesis of published data on N-linked glycosylation factors.
Main Results:
- The cell culture environment is a significant factor influencing N-linked glycosylation patterns.
- Specific environmental parameters directly affect the oligosaccharide moieties of glycoproteins.
Conclusions:
- Understanding the impact of the culture environment is crucial for optimizing protein production.
- Further research into controlling glycosylation through environmental manipulation is warranted for biopharmaceutical development.