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Related Experiment Videos

Microbial processes for ascorbic acid biosynthesis: a review.

J Boudrant1

  • 1CNRS-ENSAIA, Vandoeuvre-les-Nancy, France.

Enzyme and Microbial Technology
|May 1, 1990
PubMed
Summary

Researchers explored bacterial fermentation for L-ascorbic acid production, developing a single-step process from glucose using engineered bacteria. However, the traditional Reichstein process remains more economically viable.

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Area of Science:

  • Biotechnology
  • Microbial Chemistry

Background:

  • L-Ascorbic acid (Vitamin C) is primarily synthesized via the chemical Reichstein process.
  • Microbial fermentation offers a potential alternative for producing L-ascorbic acid precursors.

Purpose of the Study:

  • To investigate bacterial strains for efficient conversion of glucose to 2-keto-L-idonic acid, a precursor to L-ascorbic acid.
  • To develop a single-step microbial process for 2-keto-L-idonic acid production.

Main Methods:

  • Utilized identified bacterial strains capable of converting glucose to 2,5-keto-D-gluconic acid and then to 2-keto-L-idonic acid.
  • Combined bacterial strains for direct glucose to 2-keto-L-idonic acid conversion.
  • Engineered novel bacterial strains by gene transfer for a single-step fermentation process.

Main Results:

  • Achieved direct conversion of glucose to 2-keto-L-idonic acid using combined or engineered bacterial strains.
  • Demonstrated a single-step microbial transformation process for the L-ascorbic acid precursor.

Conclusions:

  • Engineered bacteria offer a potential pathway for single-step microbial synthesis of 2-keto-L-idonic acid from glucose.
  • The established Reichstein process remains the most cost-competitive method for L-ascorbic acid production currently.

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