Transient behavior of T-cell hybridomas in response to changes in metabolite concentrations in continuous culture

Effects of pulse and step changes in major metabolite concentrations such as glucose, glutamine, lactic acid, and ammonium on behavior of T-cell hybridomas in continuous culture were investigated in order to develop a strategy to maximize specific rate of lymphokine formation. Step increases in glucose, lactic acid, and ammonium concentrations decreased, and a step increase in glutamine concentration increased, the specific rate of lymphokine formation. Effects of step and pulse changes on specific rates of glucose and glutamine consumption and viability of cells were also investigated.