A method for the preparation of coimmobilizates by adhesion using polyethylenimine

A method has been described for obtaining coimmobilizates by the simultaneous binding of glucose oxidase to the cell and the enzyme-bound cell to cotton thread through adhesion using polyethylenimine (PEI). Glucose oxidase was found to adsorb onto PEI-coated yeast cells from a water suspension. The desorption observed at higher ionic strength could be obviated by cross-linking with 2% glutaraldehyde for 2 min. The enzyme-bound yeast cells could then be immobilized by adhesion on cotton thread. The coimmobilizate could be reused for over 10 batches without appreciable loss in activity.