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Updated: Feb 15, 2026

A Rapid Technique for the Visualization of Live Immobilized Yeast Cells
Published on: November 9, 2006
Continuous conversion of sucrose to fructose and gluconic acid by immobilized yeast cell multienzyme complex
1Biochemistry and Food Technology Division, Bhabha Atomic Research Centre, Bombay 400 085, India.
Abstract:
A multienzyme complex consisting of invertase, glucose oxidase, and catalase was reconstituted by binding glucose oxidase using concanavalin A (Con A) to the cell wall of Sacchararomyces cerevisiae, previously induced for maximal activities of invertase and catalase. The cell flocculate obtained was stabilized by entrapment in polyacrylamide using γ irradiation at 100 kR. This complex showed a shortening of the lag period and enhancement in gluconic acid production as compared to a similar mixture of soluble enzymes. The efficacy of the multienzyme complex has been compared with that of mixed multienzyme system composed of individually immobilized enzymes. The immobilized multienzyme complex in a continuous-flow stirred-tank reactor system could be operated for continuous conversion of sucrose to fructose and gluconic acid. The reactor system did not show any loss in efficiency in a continuous operation over 20 days.
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