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Gene expression systems for filamentous fungi.
Current Opinion in Biotechnology
|October 1, 1991
Summary
Filamentous fungi can produce high levels of extracellular protein, enabling advances in gene expression systems. This review covers recent progress in expressing fungal and non-fungal genes in improved fungal hosts.
Area of Science:
- Biotechnology
- Molecular Biology
- Mycology
Background:
- Filamentous fungi possess a high capacity for secreting extracellular proteins.
- DNA-mediated transformation has revolutionized genetic manipulation in fungi.
Purpose of the Study:
- To review recent advancements in gene expression systems for filamentous fungi.
- To highlight progress in expressing both endogenous and heterologous genes.
- To discuss improvements in fungal host strains for enhanced protein production.
Main Methods:
- Literature review of recent scientific publications.
- Analysis of developments in fungal transformation techniques.
- Synthesis of data on gene expression strategies in filamentous fungi.
Main Results:
- Significant progress has been made in developing efficient gene expression systems.
- Successful expression of diverse fungal and non-fungal genes has been achieved.
- Improvements in host strains have enhanced protein yields and secretion.
Conclusions:
- Filamentous fungi are powerful platforms for recombinant protein production.
- Ongoing research continues to refine gene expression and host capabilities.
- These advancements facilitate biotechnological applications and industrial protein manufacturing.