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Updated: Sep 13, 2026

In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
Published on: March 21, 2017
GnRH-PAP hormonotoxin targets cytotoxicity to prostate cancer cell lines
Lin Qi1, Terry M Nett, Matthew C Allen
1Department of Medicine, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Box B171, Denver, CO 80220, USA.
Abstract:
Pokeweed antiviral protein (PAP) is a plant-derived, highly potent ribosome inactivating protein that causes inhibition of protein translation and rapid cell death. We have previously described potent cytotoxic activity of a GnRH (gonadotropin-releasing hormone) receptor-targeted conjugate protein (GnRH-PAP) and demonstrated that cytotoxicity depended on the number of GnRH receptors and the duration of exposure. Here, we demonstrate that the GnRH-PAP conjugate was cytotoxic to three different prostate cancer cell lines, supporting the feasibility of using such hormonotoxins as novel therapeutics for hormone-responsive cancers such as prostate cancer.
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