Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Continuous counter-current two-phase aqueous extraction.

J B Joshi1, S B Sawant, K S Rao

  • 1Department of Chemical Technology, University of Bombay, India.

Bioseparation
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence.

Acta neuropathologica communications·2025
Same author

Mitochondria-targeted oligomeric α-synuclein induces TOM40 degradation and mitochondrial dysfunction in Parkinson's disease and parkinsonism-dementia of Guam.

Cell death & disease·2024
Same author

Mitochondria-Targeted Oligomeric α-Synuclein Induces TOM40 Degradation and Mitochondrial Dysfunction in Parkinson's Disease and Parkinsonism-Dementia of Guam.

Research square·2024
Same author

Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence.

Research square·2024
Same author

Comparison of Adsorption Performance of Biochar Derived from Urban Biowaste Materials for Removal of Heavy Metals.

Environmental management·2023
Same author

Antimicrobial Efficacy of Blended Essential Oil and Chlorhexidine against Periodontal Pathogen (<i>P.gingivalis</i>)-An <i>In Vitro</i> Study.

Nigerian journal of clinical practice·2023
Same journal

Study of the factors influencing peak asymmetry on chromatography using a molecularly imprinted polymer prepared by the epitope approach.

Bioseparation·2003
Same journal

Molecularly imprinted polymer as storage medium for an analyte.

Bioseparation·2003
Same journal

A molecular imprinted polymer with recognition properties towards the carcinogenic mycotoxin ochratoxin A.

Bioseparation·2003
Same journal

Molecular imprinting and solid phase extraction of flavonoid compounds.

Bioseparation·2003
Same journal

MIP-ligand binding assays (pseudo-immunoassays).

Bioseparation·2003
Same journal

Selective solid-phase extraction of bio- and environmental samples using molecularly imprinted polymers.

Bioseparation·2003
See all related articles

Continuous counter-current columns offer convenient protein extraction using aqueous two-phase systems. This study details design parameters for various columns, comparing their efficiency for optimal protein recovery.

Area of Science:

  • Biochemical Engineering
  • Separation Science
  • Process Design

Background:

  • Protein extraction is crucial in biotechnology and pharmaceuticals.
  • Aqueous two-phase systems (ATPS) offer a gentle method for protein partitioning.
  • Efficient contactor design is key for scalable ATPS applications.

Purpose of the Study:

  • To provide a detailed analysis of continuous counter-current column operation for protein extraction using ATPS.
  • To compare the performance of different column types (spray, packed, plate, York-Scheibel) for protein recovery.
  • To offer a step-by-step design procedure and identify future research needs.

Main Methods:

  • Detailed discussion of design parameters for spray, packed, plate, and York-Scheibel columns.

Related Experiment Videos

  • Evaluation of polymer-polymer and polymer-salt ATPS for protein partitioning.
  • Comparative analysis of operating and extraction efficiencies for various contactor types.

    • Main Results:

    • Continuous counter-current column operation simplifies contacting in ATPS for protein extraction.
    • Specific design considerations and performance data are presented for different column configurations.
    • Both polymer-polymer and polymer-salt systems demonstrate effectiveness for protein recovery.

    Conclusions:

    • Continuous column contactors are suitable for industrial-scale protein recovery via ATPS.
    • The choice of column design impacts overall extraction efficiency and operational convenience.
    • Further data are needed to refine design procedures and optimize protein recovery processes.