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Modifying the mouse: design and desire.

A Bradley1, P Hasty, A Davis

  • 1Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030.

Bio/Technology (Nature Publishing Company)
|May 1, 1992
PubMed
Summary
This summary is machine-generated.

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Creating genetically modified mice is now feasible using gene targeting in embryonic stem (ES) cells. Recent advances improve the routine generation of these valuable research models despite technical challenges.

Area of Science:

  • Genetics
  • Developmental Biology
  • Molecular Biology

Background:

  • Genetic modification of endogenous genes in mice is crucial for research.
  • Gene targeting in embryonic stem (ES) cells enables the creation of genetically altered mice.
  • Technical obstacles can hinder the routine application of these techniques.

Purpose of the Study:

  • To review recent advances in constructing genetically modified mice.
  • To discuss improvements in ES cell biology and in vitro culture.
  • To summarize techniques for isolating targeted ES cell clones and genome modification strategies.

Main Methods:

  • Application of gene targeting techniques to embryonic stem (ES) cells.
  • Generation of mice from specific ES cell clones.

Related Experiment Videos

  • Isolation and characterization of targeted ES cell clones.
  • Strategies for specific genome modification.

    • Main Results:

    • Improved understanding of ES cell biology and in vitro growth.
    • Routine germ line transmission of manipulated ES cell clones.
    • Summarization of techniques for isolating targeted clones.
    • Discussion of successful strategies for specific genome modifications.

    Conclusions:

    • Advances in ES cell technology facilitate routine generation of genetically modified mice.
    • Overcoming technical hurdles enhances the implementation of gene targeting.
    • Current strategies enable precise modifications of the mouse genome for research.