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Total solution synthesis of human epidermal growth factor (h-EGF) by the assembly of nine building blocks
S Y Shin1, Y Kaburaki, M Watanabe
1Institute of Applied Biochemistry, University of Tsukuba, Japan.
Bioscience, Biotechnology, and Biochemistry
|March 1, 1992
Abstract:
Human epidermal growth factor (h-EGF) composed of 53 amino acids bearing three intramolecular disulfide bridges was synthesized by the maximum protecting solution method. The synthetic h-EGF coincided with recombinant h-EGF by reverse-phase HPLC, and the sites of three intramolecular disulfide bridges were ascertained by a thermolytic digestion. The synthetic h-EGF possessed m/z 6215.7 in its FAB-MS as expected, and exhibited compatible mitogenic activity.