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Related Experiment Videos

Ceramic membrane microfilter as an immobilized enzyme reactor.

T J Harrington1, J L Gainer, D J Kirwan

  • 1Department of Chemical Engineering, University of Virginia, Charlottesville 22903.

Enzyme and Microbial Technology
|October 1, 1992
PubMed
Summary

This study shows ceramic microfilters effectively immobilize enzymes for bioreactors. Enzyme kinetics were unaffected by flow rates, indicating no mass transfer or shear effects, making them a promising alternative reactor design.

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Area of Science:

  • Biochemical Engineering
  • Materials Science

Background:

  • Immobilized enzymes are crucial for biocatalysis and industrial processes.
  • Ceramic microfilters offer a porous matrix for enzyme immobilization.
  • Understanding reactor performance is key to optimizing enzyme applications.

Purpose of the Study:

  • To evaluate ceramic microfilters as immobilized enzyme reactors.
  • To investigate the impact of permeation rate on enzyme kinetics.
  • To assess potential mass transfer or shear effects in ceramic microfilter reactors.

Main Methods:

  • Enzymes (penicillinase, lactate dehydrogenase) were immobilized within a ceramic microfilter.
  • Substrate solutions were permeated through the microfilter at varying rates.
  • Kinetic parameters were measured to assess enzyme performance.

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  • Transmembrane pressure drop was regulated to control residence time and conversion.

    • Main Results:

    • Observed kinetic parameters remained independent of flow rate for immobilized penicillinase and lactate dehydrogenase.
    • No significant mass transfer or shear effects were detected.
    • Residence time and conversion were effectively controlled by transmembrane pressure drop.

    Conclusions:

    • Ceramic microfilter reactors are suitable for immobilized enzyme applications.
    • They offer an alternative to packed bed reactors, particularly for highly active enzymes with low Michaelis constants.
    • The reactor design allows for straightforward control over reaction conditions.